Real-Time PCR System - 6-Channel qPCR Thermal Cycler - 96 Well - 8 °C/s Ramp

The Master Science 6-Channel Real-Time PCR runs quantitative PCR on 96 wells with six fluorescence channels read at the same time. A side-scan optical design collects all channels together and scans a full plate in 5 seconds, giving strong signals with low background.

Fast block ramping up to 8 °C/s, a 0.1 to 42 °C gradient and an automatic hot lid support qualitative and absolute quantification, relative quantification, genotyping, allele identification, HRM and melting curve analysis.

Technical Specifications

Specifications of the Master Science 6-Channel Real-Time PCR:

• Brand: Master Science
• Model Number: MSPCR-Q96-6PSWISS
• SKU: MSPCR-Q96-6PSWISS
• Brand: Master Science
• Model Number: MSPCR-Q96-6PSWISS
• SKU: MSPCR-Q96-6PSWISS
• Sample Capacity: 96-well plate, 12 × 8-tube strips or 96 × 0.2 mL tubes
• Consumables: 0.1/0.2 mL tubes, 8-tube strips, skirtless and semi-skirted 96-well plates (0.2 mL recommended)
• Reaction Volume: 10 to 100 µL
• Temperature Range: 0 to 100 °C, 0.1 °C resolution
• Maximum Ramp Rate: 8.0 °C/s
• Control Accuracy: ±0.1 °C
• Uniformity / Accuracy: ±0.2 °C / ±0.2 °C
• Gradient Span: 0.1 to 42 °C
• Hot Lid: Automatic, 30 to 115 °C (105 °C default)
• Temperature Control Mode: Block or tube
• Detection Channels: 6, scanned simultaneously
• Excitation / Emission: 300 to 810 nm / 500 to 810 nm
• Light Source / Detector: Long-life LED / high-sensitivity photodetector
• Scan Time: 5 s for 96 wells
• Calibrated Dyes: FAM/SYBR Green/EvaGreen; HEX/VIC/JOE/TET/Yellow; ROX/Texas Red; Cy5; Cy5.5; Cy3 (custom)
• Dynamic Range: 1 to 10¹⁰ copies
• Sensitivity: 1 copy
• Control: 12.1-inch colour touch screen or PC
• Data Export: Excel, CSV, TXT, PDF; direct report printing; LIMS connection
• Interfaces: USB 2.0, RS232
• Power Supply: 100 to 240 V AC, 50/60 Hz, 1500 W
• Dimensions (L × W × H): 520 × 350 × 330 mm
• Weight: 32 kg

Key Benefits

• Six targets per well: the Master Science 6-Channel Real-Time PCR reads six dyes together.
• Fast plates: 5-second scans and 8 °C/s ramping.
• Assay optimisation: gradient across 0.1 to 42 °C.
• Automation ready: automatic hot lid for workstations.
• Flexible control: touch screen or one PC for several units.

Prepare templates with the Master Science automatic nucleic acid extractor.

Product Features

Optics

• 6-channel simultaneous side scanning
• LED excitation
• 1-copy sensitivity

Thermal

• 8 °C/s ramp
• ±0.1 °C control
• 0.1 to 42 °C gradient

Software and Control

• Quantification, HRM, genotyping
• 12.1-inch touch screen or PC
• LIMS and automation connectivity

Applications

The Master Science 6-Channel Real-Time PCR is used for:

• Qualitative and absolute quantification
• Relative gene expression
• Genotyping and allele identification
• High-resolution melting (HRM)
• Melting curve analysis

Common Laboratory Problems and How This System Helps

How the Master Science 6-Channel Real-Time PCR improves qPCR work:

• Too few channels for multiplex assays: six detection channels.
• Slow runs: fast ramping and 5 s scans.
• Annealing temperature guesswork: built-in gradient.
• Manual lid handling in automated labs: automatic hot lid.