LED Fluorescence Microscope - Trinocular Epi-Fluorescence Microscope - 6-Position Turret, FN 23

The Master Science Fluorescence Microscope is an upright research microscope built around an infinity optical system and a broadband high-power LED fluorescence source. The LED covers 350 to 760 nm, needs no warm-up and can be switched on and off at will, so fluorescence work starts straight away.

A six-position turret carries the filter cubes, with blue, green and ultraviolet excitation sets fitted as standard. Besides fluorescence, the Master Science Fluorescence Microscope supports brightfield, phase contrast and darkfield observation, and a light intensity manager keeps brightness matched as objectives are changed.

Technical Specifications

Specifications of the Master Science Fluorescence Microscope are given below.

• Brand: Master Science
• Model Number: MSMSC-F43NSWISS
• SKU: MSMSC-F43NSWISS
• Optics: Infinity optical system
• Observation Tube: Tilting trinocular, interpupillary distance 50-75 mm
• Viewing Head: Siedentopf head, inclined at 30°
• Light Split Ratio (Binocular/Trinocular): 100:0 / 20:80 / 0:100
• Eyepiece: Wide field 10X/23 mm; 10X/25 mm (optional)
• Objectives: Plan semi-apochromatic 4X, 10X, 40X, 100X; 20X (optional)
• Epi-fluorescence Light Source: Wide-spectrum high-power LED fluorescence source (standard); 4-channel light source (optional); touch-screen controller
• Epi-fluorescence Illuminator: 6-position turret; B, G, U three channels standard; Y, V, R and others optional
• U Filter Set: EX 375/30 nm; DM 415 nm; EM 460/50 nm
• B Filter Set: EX 475/30 nm; DM 505 nm; EM 530/40 nm
• G Filter Set: EX 540/25 nm; DM 565 nm; EM 605/55 nm
• V Band (optional): EX 405/30 nm; DM 440 nm; EM 450 nm LP
• R Band (optional): EX 620/50 nm; DM 655 nm; EM 692/45 nm
• Y Band (optional): EX 560/40 nm; DM 600 nm; EM 610 nm LP
• Dual-band B/G (optional): EX 470/40 and 575/35 nm; EM 525/40 and 625/60 nm
• Royal Blue B2 (optional): EX 450/50 nm; DM 485 nm; EM 495 nm LP
• Yellow G2 (optional): EX 560/40 nm; DM 600 nm; EM 610 nm LP
• Nosepiece: Inward quintuple nosepiece
• Stage: Highly wear-resistant ceramic-coated stage; moving range 80 x 50 mm; precision 0.1 mm
• Focusing: Coaxial coarse and fine adjustment; 25 mm stroke; coarse upper limit stop; adjustable coarse torque; fine scale 1 μm
• Transmitted Illumination: Abbe condenser N.A. 1.1, Kohler illumination; warm white LED, continuously adjustable, with light intensity manager

Key Benefits

• Ready immediately:: the LED source of the Master Science Fluorescence Microscope needs no preheating.
• Six filter positions:: cubes are exchanged without tools.
• Consistent brightness:: light intensity manager sets the level for each objective.
• Comfortable for long sessions:: low-position stage and focus controls.
• Four contrast methods:: brightfield, fluorescence, phase contrast and darkfield.

For routine brightfield research work, see the Master Science semi-apochromatic biological microscope.

Product Features

Ergonomics

• Low-torque, low-position fixed stage and focusing mechanism reduce hand movement and fatigue
• Wide-field trinocular tube inclined at 30°
• Light intensity management sets matching brightness for each objective automatically

Fluorescence System

• Long-life broadband high-power LED with uniform brightness and minimal maintenance
• Spectral coverage from 350 nm to 760 nm, on/off without preheating
• Six-position fluorescence module with band-pass filter sets, replaceable without tools
• Semi-apochromatic objectives with high fluorescence sensitivity for sharp, high-contrast images

Observation Methods

• Brightfield: condenser for continuous 20X-100X (oil) observation with a flat field
• Fluorescence: user-replaceable filter cubes and high signal-to-noise ratio, suited to FISH, FRET and CTC detection
• Phase contrast: with a phase contrast condenser, for internal cell structures and live bacteria
• Darkfield: detail reproduction from low to high magnification

Applications

The Master Science Fluorescence Microscope is used for fluorescence and multi-mode observation in:

• FISH, FRET and CTC detection
• Cell biology and live bacteria observation
• Biological tissue examination
• Research and teaching laboratories

Optional Accessories

• 10X/25 mm eyepiece
• 20X plan semi-apochromatic objective
• 4-channel fluorescence light source
• Additional filter sets (V, R, Y, dual-band B/G, B2, G2)

Common Problems This Microscope Solves

Fluorescence microscopy with older lamp systems brings familiar frustrations.

• Waiting for a lamp to warm up:: the LED source works the moment it is switched on.
• Re-adjusting brightness at every objective change:: the intensity manager does it automatically.
• Tools needed to swap filter cubes:: the turret cubes change by hand.
• Tired hands and shoulders:: controls sit low and close together.