Inverted Biological Microscope - LED Phase Contrast Cell Culture Microscope - 4X-40X LWD, FN 22

The Master Science Inverted Biological Microscope is built on an infinity optical system for viewing cultures from underneath, directly in their vessels. It suits live-cell imaging, transparent liquid tissues and day-to-day monitoring of cultures in dishes and flasks.

Long working distance plan objectives, a push-pull phase contrast slider and a long-life LED lamp make the Master Science Inverted Biological Microscope quick to set up. Its compact, integrated body fits inside laminar flow hoods, and a built-in 0.75X C-mount on the lower left of the base keeps the camera out of the working area.

Technical Specifications

Specifications of the Master Science Inverted Biological Microscope are given below.

• Brand: Master Science
• Model Number: MSMSC-IV52BNSWISS
• SKU: MSMSC-IV52BNSWISS
• Optics: Infinity optical system
• Observation Tube: Tilting trinocular, interpupillary distance 53-75 mm, light split 100:0 / 0:100
• Viewing Head: Siedentopf head, inclined at 45°
• Eyepiece: Wide field WF10X/22 mm; centering telescope
• Objective 4X: Infinity LWD plan achromatic, Plan 4X/0.12, W.D. 10.8 mm
• Objective 10X PH: Infinity LWD plan achromatic phase contrast, Plan 10X/0.25 PH, W.D. 4.1 mm
• Objective 20X PH: Infinity LWD plan achromatic phase contrast, Plan 20X/0.45 PH, W.D. 5 mm
• Objective 40X: Infinity LWD plan achromatic, LACH Plan 40X/0.60, W.D. 3.5 mm
• Nosepiece: Quintuple nosepiece, ball-bearing, with anti-fungus treatment
• Focusing: Coaxial coarse and fine focusing with tension adjustment and upper limit stop; fine graduation 2 μm
• Stage: Fixed stage 227 x 208 mm; mechanical stage with 135 x 77 mm travel
• Glass Round Stage Plate: Outer φ118 mm, inner φ68 mm
• Petri Dish Holder 1: Locating slot 86 x 129.5 mm, for φ90 mm round dishes
• Petri Dish Holder 2: Locating slot 34 x 77.5 mm, for φ68.5 mm round dishes
• Petri Dish Holder 3: Locating slot 57 x 82 mm, for φ60 mm round dishes
• Petri Dish Holder 4: Locating slot 29 x 77.5 mm, for φ35 mm round dishes
• Transmitted Illumination: White LED, adjustable brightness; push-pull condenser, working distance 55 mm; green filter
• Phase Contrast Ring Plate: 10X, 20X, 40X (20X and 40X in one unit)
• Camera Port: Built-in 0.75X C-mount (camera not included)
• External Dimensions (L x W x H): 486 x 255 x 537 mm

Key Benefits

• Cultures stay in their vessels:: the Master Science Inverted Biological Microscope views dishes and flasks from below.
• Unstained cells made visible:: push-pull phase contrast slider.
• Any common dish size:: four quick-release Petri dish holders.
• Comfortable long sessions:: 45° head and 22 mm high eye point eyepieces.
• Fits in a flow hood:: compact integrated body with a rear carrying design.

For slide-based work on an upright stand, see the Master Science trinocular biological microscope.

Product Features

Viewing

• Siedentopf head inclined at 45° with 53-75 mm interpupillary range
• 22 mm wide-field, high eye point eyepieces with adjustable diopters
• Usable while wearing glasses

Illumination and Imaging

• Long-life LED: stable, uniform, glare-free light with continuously adjustable intensity
• Push-pull phase contrast slider for quick phase observation
• Built-in 0.75X C-mount adapter compatible with most mainstream digital cameras
• Camera port on the lower left of the base leaves the workspace clear

Body and Stage

• Integrated, space-saving modular design for laminar flow hoods and small laboratories
• Portable rear design for safe relocation
• Quick-release sample holder with several Petri dish holders
• Flat stage surface for irregularly shaped dishes

Applications

The Master Science Inverted Biological Microscope is ideal for:

• Live-cell imaging
• Transparent liquid tissues
• Dynamic monitoring of cultures in their vessels
• Cell culture laboratories

Optional Accessories

• 3-channel digital LED fluorescence attachment
• Plan Fluor 10X/20X/40X PH long working distance phase contrast objectives

Common Problems This Microscope Solves

Checking cell cultures on an ordinary microscope is awkward for several reasons.

• Flasks and dishes do not fit under an upright objective:: the objectives sit below the stage.
• Living cells are nearly invisible in brightfield:: phase contrast shows them without staining.
• Dishes slide about on the stage:: matched holders lock each size in place.
• No space in the hood:: the compact body is designed to work inside it.